{"id":829,"date":"2025-07-11T00:01:12","date_gmt":"2025-07-11T00:01:12","guid":{"rendered":"https:\/\/www-user.yokohama-cu.ac.jp\/~aritak7225\/?p=829"},"modified":"2025-07-11T00:01:12","modified_gmt":"2025-07-11T00:01:12","slug":"usp7-in-action-cryo-em-captures-the-structural-change-of-usp7","status":"publish","type":"post","link":"https:\/\/arita-lab.com\/en\/2025\/07\/11\/usp7-in-action-cryo-em-captures-the-structural-change-of-usp7\/","title":{"rendered":"USP7 in Action! Cryo-EM Captures the structural change of USP7."},"content":{"rendered":"\n<p class=\"wp-block-paragraph\">A research paper by Nao Nakamura (M2) and Sae Yoshimi (Master\u2019s graduate, FY2022) has been published in the journal <em>Structure<\/em>, highlighting new insights into the activation mechanism of the deubiquitinase USP7.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">USP7 plays a key role in DNA maintenance methylation by removing ubiquitin from histone H3. In this study, we developed an <em>in vitro<\/em> assay system to evaluate the deubiquitination activity of USP7 and conducted a series of biochemical experiments to dissect the reaction in detail.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Using cryo-EM, we successfully visualized USP7 in complex with DNMT1, revealing two distinct structural states: an open, inactive conformation and a closed, active conformation. These findings provide a molecular basis for how USP7 becomes activated and highlight the dynamic nature of its function.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n","protected":false},"excerpt":{"rendered":"<p>A research paper by Nao Nakamura (M2) and Sae Yoshimi (Master\u2019s graduate, FY2022) has been published in the jo [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":1022,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"swell_btn_cv_data":"","_locale":"en_US","_original_post":"https:\/\/www-user.yokohama-cu.ac.jp\/~aritak7225\/?p=828","footnotes":""},"categories":[8],"tags":[],"class_list":["post-829","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-research","en-US"],"_links":{"self":[{"href":"https:\/\/arita-lab.com\/wp-json\/wp\/v2\/posts\/829","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/arita-lab.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/arita-lab.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/arita-lab.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/arita-lab.com\/wp-json\/wp\/v2\/comments?post=829"}],"version-history":[{"count":0,"href":"https:\/\/arita-lab.com\/wp-json\/wp\/v2\/posts\/829\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/arita-lab.com\/wp-json\/wp\/v2\/media\/1022"}],"wp:attachment":[{"href":"https:\/\/arita-lab.com\/wp-json\/wp\/v2\/media?parent=829"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/arita-lab.com\/wp-json\/wp\/v2\/categories?post=829"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/arita-lab.com\/wp-json\/wp\/v2\/tags?post=829"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}